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http://krishi.icar.gov.in/jspui/handle/123456789/9560| Title: | Genotyping of Indian antigenic, vaccine and field Brucella sp. using Multiple Locus Sequence Typing |
| Other Titles: | Not Available |
| Authors: | Shome R Krithiga N Padmashree BS Shome BR Saikia GK N.S. Chauhan SH Chandel BS Rajendhran J Rahman H |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2016-03-31 |
| Project Code: | Not Available |
| Keywords: | Genotyping Indian antigenic vaccine field Brucella sp. Multiple Locus Sequence Typing |
| Publisher: | Journal of Infection in developing Countries |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | INTRODUCTION: Brucellosis is one of the most important zoonotic diseases that affects multiple livestock species and causes great economic losses. The highly conserved genomes of Brucella, with > 90% homology among species, makes it important to study the genetic diversity circulating in the country. METHODOLOGY: A total of 26 Brucella spp. (4 reference strains and 22 field isolates) and 1 B. melitensis draft genome sequence from India (B. melitensis Bm IND1) were included for sequence typing. The field isolates were identified by biochemical tests and confirmed by both conventional and quantitative polymerase chain reaction (qPCR) targeting bcsp 31Brucella genus-specific marker. Brucella speciation and biotyping was done by Bruce ladder, probe qPCR, and AMOS PCRs, respectively, and genotyping was done by multilocus sequence typing (MLST). RESULTS: The MLST typing of 27 Brucella spp. revealed five distinct sequence types (STs); the B. abortus S99 reference strain and 21 B. abortus field isolates belonged to ST1. On the other hand, the vaccine strain B. abortus S19 was genotyped as ST5. Similarly, B. melitensis 16M reference strain and one B. melitensis field isolate were grouped into ST7. Another B. melitensis field isolate belonged to ST8 (draft genome sequence from India), and only B. suis 1330 reference strain was found to be ST14. CONCLUSION: The sequences revealed genetic similarity of the Indian strains to the global reference and field strains. The study highlights the usefulness of MLST for typing of field isolates and validation of reference strains used for diagnosis and vaccination against brucellosis. |
| Description: | Not Available |
| ISSN: | 1972-2680 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Infection in Developing Countries |
| NAAS Rating: | 6.7 |
| Volume No.: | 10(3) |
| Page Number: | 237-44 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.3855/jidc.6617 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/9560 |
| Appears in Collections: | AS-NIVEDI-Publication |
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