KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/9566
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Patil SS | en_US |
dc.contributor.author | Prajapati A | en_US |
dc.contributor.author | Hemadri D | en_US |
dc.contributor.author | Suresh KP | en_US |
dc.contributor.author | Desai GS | en_US |
dc.contributor.author | Reddy GBM | en_US |
dc.contributor.author | Chandranaik BM | en_US |
dc.contributor.author | Ranganatha S | en_US |
dc.contributor.author | Rahman H | en_US |
dc.date.accessioned | 2018-11-06T08:40:26Z | - |
dc.date.available | 2018-11-06T08:40:26Z | - |
dc.date.issued | 2016-12-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 2307-8316 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/9566 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Aim: This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease. Materials and Methods: Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software. Results: A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain. Conclusion: BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Veterinary World | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | bovine herpesvirus 1-1 | en_US |
dc.subject | glycoprotein B | en_US |
dc.subject | India | en_US |
dc.subject | infectious bovine rhinotracheitis | en_US |
dc.subject | phylogenetic analysis | en_US |
dc.subject | sequencing | en_US |
dc.subject | subtype 1.1 | en_US |
dc.title | Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Veterinary World | en_US |
dc.publication.volumeno | 9(12) | en_US |
dc.publication.pagenumber | 1364-1369 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.14202/vetworld.2016.1364-1369 | en_US |
dc.publication.authorAffiliation | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics | en_US |
dc.publication.authorAffiliation | Institute of Animal Health and Veterinary Biologicals, Bengaluru | en_US |
dc.publication.authorAffiliation | Indian Council of Agricultural Research, New Delhi | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | Not Available | en_US |
Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
File | Description | Size | Format | |
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VetWorld-9-1364.pdf | 1.55 MB | Adobe PDF | View/Open |
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