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Formation of Bowman–Birk inhibitors during the germination of horsegram (Dolichos biflorus)

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Relation http://ir.cftri.com/1426/
Phy_06_02
 
Title Formation of Bowman–Birk inhibitors during the
germination of horsegram (Dolichos biflorus)
 
Creator Kumar, Pradeep
Sreerama, Yadahalli N.
Gowda, Lalitha R.
 
Subject 29 Protein Chemistry
22 Legumes-Pulses
 
Description Three Bowman–Birk type inhibitors (HGGI-I, II and III), which appear in the cotyledons of 120 h germinated horsegram (Dolichos biflorus) seeds have been purified to homogeneity by size-exclusion chromatography and ion-exchange chromatography. HGGI-I, HGGI-II and HGGI-III differ from each other and from the dormant seed inhibitors in amino acid composition, molecular size and charge. The amino-terminal sequence analyses indicate that these inhibitors are derived from the isoinhibitors of the
dormant seed by a limited proteolysis and not by de novo synthesis. These inhibitors differ from each other at their amino-terminus. HGGI-II identical to HGGI-I except for the loss of a single amino-terminal aspartyl residue, where as HGGI-III shows the loss of a pentapeptide. All the three inhibitors are potent competitive inhibitors of trypsin and chymotrypsin. The dissociation constants (Kis)
for trypsin inhibition indicate that amino-terminal tail of the inhibitors play a role in trypsin binding probably through electrostatic interaction.
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1426/1/Phytochemistry_60_%282002%29_581-588.pdf
Kumar, Pradeep and Sreerama, Yadahalli N. and Gowda, Lalitha R. (2002) Formation of Bowman–Birk inhibitors during the germination of horsegram (Dolichos biflorus). Phytochemistry, 60. pp. 581-588.