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In vitro multiplication of Vanilla planifolia using axillary bud explants

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1431/
PCR_01_97
 
Title In vitro multiplication of Vanilla planifolia using axillary bud explants
 
Creator George, P. S.
Ravishankar, G. A.
 
Subject 10 Plants
05 Tissue Culture
 
Description A clonal propagation method has been developed
for efficient multiplication of Vanilla planifolia. Multiple shoots were developed from axillary bud explants
using semi-solid Murashige and Skoog (MS) medium
supplemented with N6-benzyladenine (BA, 2 mg l–1) and
a-naphthaleneacetic acid (NAA, 1 mg l–1). The multiple
shoots were transferred to agitated liquid MS medium with
BA at 1 mg l–1 and NAA at 0.5 mg l–1 for 2–3 weeks, and
subsequently cultured on semi-solid medium. Using this
method, an average of 42 shoots were obtained from a single
axillary bud explant over a period of 134 days. Use of
an intervening liquid medium has been found to enhance
multiplication of shoots in V. planifolia.
 
Date 1997
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1431/1/Plant_Cell_Reports_16%281997%29_490-494.pdf
George, P. S. and Ravishankar, G. A. (1997) In vitro multiplication of Vanilla planifolia using axillary bud explants. Plant Cell Reports, 16. pp. 490-494.