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Influence of putrescine, silver nitrate and polyamine inhibitors on the morphogenetic response in untransformed and transformed tissues of Cichorium intybusand their regenerants

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Title Influence of putrescine, silver nitrate and polyamine inhibitors on the morphogenetic response in untransformed
and transformed tissues of Cichorium intybusand their regenerants
 
Creator Bais, H. P.
Sudha, G.
Ravishankar, G. A.
 
Subject 10 Plants
05 Tissue Culture
 
Description The addition of 40 mM putrescine (Put) to
Murashige and Skoog’s (MS) medium resulted in increased
shoot multiplication and shoot growth in untransformed
plants relative to transformed plants of
Cichorium intybus L. Put at a concentration of 40 mM
also resulted in flowering in both systems on the 28th
day, with elevated titers of endogenous conjugated Put
and spermine (Spm) in both untransformed and transformed
plants. The addition of 40 µM AgNO3 to untransformed
axillary buds of C. intybus L. cultured on MS
media resulted in increased shoot multiplication (36.9±
2.63 shoots per culture) and increased shoot growth
(7.82±0.76 cm) as compared to transformed ones (11.6±
0.89 shoots per culture; 3.20±0.24 cm). Moreover, cultures
treated with 40 µM AgNO3 showed in vitro flowering
on the 28th day in both systems, with the endogenous
levels of conjugated spermine being higher in untransformed
plants than in transformed ones. The morphogenetic
response and the endogenous conjugated pool of polyamines were lower following á-DL-difluromethylarginine
and á-DL-difluromethylornithine treatments;
the addition of put (40 mM) and AgNO3 (40 µM)
restored these to normal levels. Under exogenous put
feeding, ethylene production was lower in both the untransformed and transformed cultures. We believe that an
interplay between polyamine and ethylene biosynthesis
is involved in regulating the morphogenetic response in
both transformed and untransformed shoots of C. intybus.
The response to AgNO3 and Put treatment was not
altered by the transformation process.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1432/1/Plant_Cell_Reports_20%282001%29_547-555.pdf
Bais, H. P. and Sudha, G. and Ravishankar, G. A. (2001) Influence of putrescine, silver nitrate and polyamine inhibitors on the morphogenetic response in untransformed and transformed tissues of Cichorium intybusand their regenerants. Plant Cell Reports, 20. pp. 547-555.