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Effect of abscisic acid and cytokinins on cultured zygotic embryos of Coffea arabica cv.Cauvery (Catimor)

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PCT_02_04
 
Title Effect of abscisic acid and cytokinins on cultured zygotic embryos of Coffea arabica cv.Cauvery (Catimor)
 
Creator Madhava Naidu, M.
Sreenivasan, C.S.
 
Subject 02 Plant Biochemistry
04 Coffee
 
Description Immature zygotic embryos of Coffea arabica L.cv.Cauvery (Catimor) were sequentially cultured on different
modifications of Murashige and Skoog’s (MS) medium to test the effect of abscisic acid and cytokinins.
The type of response depended on the medium strength, the growth regulator combinations as well
as the period of initial culture in both abscisic acid or cytokinin supplemented media. Increasing concentration
of abscisic acid from 0.4 to 18.9 lM enhanced the quiescence of the zygotic embryos. All the
cytokinins promoted germination but Kinetin and isopentenyladenine (2-IP) were less effective than benzyl
amino purine (BAP). The maximum mature embryos were obtained when immature embryos were cultured
initially for 30 days on full strength MS medium with 3.8 lM ABA, followed by 60 days on half strength
MS medium with 0.1 lM BAP and finally on half strength MS media with out growth regulator for next
60 days.
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1434/1/Plant_Cell%2C_Tissue_and_Organ_Culture_79_279-284.pdf
Madhava Naidu, M. and Sreenivasan, C.S. (2004) Effect of abscisic acid and cytokinins on cultured zygotic embryos of Coffea arabica cv.Cauvery (Catimor). Plant Cell Tissue and Organ Culture, 79. pp. 279-284.