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Interplay of growth regulators during solid-state and liquid-state batch cultivation of anthocyanin producing cell line of Daucus carota

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PC_40_05
 
Title Interplay of growth regulators during solid-state and liquid-state batch cultivation of anthocyanin producing cell line of Daucus carota
 
Creator Narayan, M. S.
Thimmaraju, R.
Bhagyalakshmi, N.
 
Subject 04 Carrot
01 Plant Cell
 
Description The influence of different growth regulators on biomass (BM) accumulation and anthocyanin (AC) content in solid-state and liquid-state batch cultures of Daucus carota was studied. While all the auxins such as 2,4-D, IAA and NAA supplemented at different levels, supported
growth as well as AC synthesis, the maximum productivity of AC (1.27 g l−1) was observed in the presence of 2.5 mg l−1 of IAA followed by 1mg l−1 of NAA (0.5 g l−1of AC). Among the cytokinins, kinetin (0.1 and 0.2 mg l−1) supported highest AC productivity. The interplay
of different levels of IAA and kinetin revealed that the combination of IAA at 2.5 mg l−1 and kinetin at 0.2 mg l−1 was superior to other combinations both in solid-state as well as liquid-state cultures, where AC productivity in solid-state was much higher (five-fold) than that in
liquid-state. Long term effects of the best IAA and NAA levels indicated that only IAA could support uniform productivity of AC in solid-state only. In liquid-state cultures, AC synthesis dropped steadily upto six subcultures where the subsequent increase of IAA by 0.5 mg l−1 brought back the level of AC for a limited number of subsequent subcultures. Increase of cytokinin did not improve AC productivity. Temperature also
imparted significant effect on AC productivity with 30 ◦C being for solid-state whereas for liquid-state 25 ◦C was the best. In general, highest and stable production of AC occurred only in solid-state rather than in liquid-state, though the latter is generally preferred for automation of
scaled-up production.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/1486/1/Process_Biochemistry_40_%282005%29_351-358.pdf
Narayan, M. S. and Thimmaraju, R. and Bhagyalakshmi, N. (2005) Interplay of growth regulators during solid-state and liquid-state batch cultivation of anthocyanin producing cell line of Daucus carota. Process Biochemistry, 40. pp. 351-358.