ICAR Research Data Repository for Knowledge Management
Single Step Method of Preparation of Detergent- Permeabilized Kluyveromyces fragilis for Lactose Hydrolysis
IR@CSIR-CFTRI
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/1489/
PC_42_96 |
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| Title |
Single Step Method of Preparation of Detergent- Permeabilized Kluyveromyces fragilis for Lactose Hydrolysis |
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| Creator |
Bachhawat, N.
Gowda, L. R. Bhat, S. G. |
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| Subject |
19 Yeast
05 Carbohydrate Biochemistry |
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| Description |
β-Galactosidase activity of CTAB-permeabilized K. fragilis depends upon the cell to detergent ratio and the pH of the permeabilizing medium. Maximum β-galactosidase activity was observed when the cell to detergent ratio was I: 001 at neutralpH. A simple and single step methodfor the preparation of whole cell β-galactosidase is described which involves adjusting the pH of the growth medium at the beginning of the stationary phase to 7.0, addition of CTA R directly to the growth medium at 1% concentration of biomass (wet weight), before harvesting cells by centrifugation. This whole cell enzyme activity readily hydrolysed high concentrations of lactose in buffer and whey. |
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| Date |
1996
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/1489/1/Process_Biochemistry_Vol._31%2C_No._I%2C_21-25%2C_1996.pdf
Bachhawat, N. and Gowda, L. R. and Bhat, S. G. (1996) Single Step Method of Preparation of Detergent- Permeabilized Kluyveromyces fragilis for Lactose Hydrolysis. Process Biochemistry, 31 (1). pp. 21-25. |
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