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Screening and selection of media components for lactic acid production using Plackett-Burman design.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1494/
http://www.springerlink.com/content/9an2cp8jd98fdq3u/?p=e90aaf2b68514c54b1c7ad944648cf52&pi=9
10.1007/PL00009003
 
Title Screening and selection of media components for lactic acid production using Plackett-Burman design.
 
Creator Krishnan, S.
Prapulla, S. G.
Rajalakshmi, D.
Misra, M. C.
Karanth, N. G.
 
Subject 27 Dairy products
 
Description Plackett-Burman design was used to efficiently select important media components influencing lactic acid production in a two step screening procedure. A total of 36 screening experiments were conducted for studying the effect of various media components such as carbon and nitrogen (simple and complex) sources, minerals/buffering agents and a specific inducer for the production of lactic acid by Lactobacillus plantarum NCIM 2084. The eleven ingredients chosen after the first screening experiments were further screened by a Plackett-Burman design consisting of 12 experiments. Liquefied starch, wheat bran extract, ammonium nitrate, manganese sulphate and sodium acetate were chosen as promising ingredients for further optimisation studies. The highest yield of 41.9 g/l of lactic acid was obtained at the end of 24 hours of fermentation which corresponded to 90% conversion, on the basis of sugar supplied.
 
Date 1998
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1494/1/Bioprocess_and_Biosystems_Engineering_19%281998%29_61-65.pdf
Krishnan, S. and Prapulla, S. G. and Rajalakshmi, D. and Misra, M. C. and Karanth, N. G. (1998) Screening and selection of media components for lactic acid production using Plackett-Burman design. Bioprocess and Biosystems Engineering, 19 (1). pp. 61-65. ISSN 1615-7591