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Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite

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T_05_05
 
Title Morphological and biochemical perturbations
in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite
 
Creator Prasanthi, K.
Muralidhara, Dr.
Rajini, P.S.
 
Subject 17 Toxicology
 
Description Erythrocytes are a convenient model to understand the membrane oxidative damage induced by various xenobiotic-prooxidants.In this investigation, we have examined the potency of Fenvalerate (FEN) and its metabolite, p-chlorophenyl isovaleric acid (p-CPIA) to induce oxidative stress response in rat erythrocytes in vitro in terms of lipid peroxidation and effects on selected antioxidant
enzymes. Susceptibility of erythrocytes to FEN exposure was further investigated in terms of morphological alterations by scanning electron microscopy and protein damage by gel electrophoresis of erythrocyte ghosts. Following in vitro exposure,FEN caused a significant induction of oxidative damage in erythrocytes at concentrations beyond 0.1 mM as evidenced by increased
thiobarbituric acid reactive substances (TBARS) levels. The response was both concentration and time dependent. At higher concentrations,significant decreases in the activities of vital antioxidant enzymes viz., catalase, superoxide dismutase, glutathione transferase
and glutathione reductase were also discernible clearly suggesting the potency of both, parent compound and its metabolite to induce oxidative stress in erythrocytes. Scanning electron micrographs of erythrocytes following FEN exposure at higher concentrations revealed various degrees of distortion in shape and ruptured membranes. Furthermore, gel electrophoresis studies revealed
consistent and significant aggregation of only band 3 protein in erythrocyte membranes exposed to either FEN or p-CPIA at higher concentrations. These in vitro findings show that FEN and its metabolite have the propensity to cause significant oxidative damage in rat erythrocytes, which is associated with marked damage to membrane proteins. These data suggest that both structural and
functional perturbations may ensue in erythrocytes following exposure to FEN at higher concentrations under in vivo situations.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1499/1/Toxicology_in_Vitro_19_%282005%29_449-456.pdf
Prasanthi, K. and Muralidhara, Dr. and Rajini, P.S. (2005) Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite. Toxicology in Vitro, 19. pp. 449-456.