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Purifcation and characterization of a platelet aggregation inhibitor acidic phospholipase A2 from Indian saw-scaled viper (Echis carinatus ) venom

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T_06_99
 
Title Purifcation and characterization of a platelet
aggregation inhibitor acidic phospholipase A2
from Indian saw-scaled viper (Echis carinatus )
venom
 
Creator Kemparaju, K.
Krishnakantha, T. P.
Veerabasappa Gowda, T.
 
Subject 07 Enzyme Biochemistry
 
Description An acidic phospholipase A2 (EC-I-PLA2) has been puri®ed from the Indian saw-scaled viper (Echis carinatus ) venom through a combination of column chromatography and
electrophoresis. EC-I-PLA2 has a molecular weight of 16000 by SDS-PAGE. It was focussed between pH 4.2 and 4.8 by isoelectro focussing. EC-I-PLA2 was non-lethal to mice
and devoid of neurotoxicity, myotoxicity, anticoagulant activity and cytotoxicity. It induced mild oedema in the foot pads of mice. The puri®ed PLA2 inhibited ADP, collagen and epinephrine induced human platelet aggregation and the inhibition was both dose and time
dependent.
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1500/1/Toxicon_37_%281999%29_1659-1671.pdf
Kemparaju, K. and Krishnakantha, T. P. and Veerabasappa Gowda, T. (1999) Purifcation and characterization of a platelet aggregation inhibitor acidic phospholipase A2 from Indian saw-scaled viper (Echis carinatus ) venom. Toxicon, 37. pp. 1659-1671.