Record Details

Investigation on the Production of Food Ingredients and Biotransformation Using Hairy Root Cultures

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/1532/
 
Title Investigation on the Production of Food Ingredients and Biotransformation Using Hairy Root Cultures
 
Creator Suresh, B.
 
Subject 08 Food technology
01 Biotechnology and Bioengineering
05 Tissue Culture
 
Description The study throws light on the production of betalaines from hairy roots of B. vulgaris and biotransformations using C. frutescens normal roots for the production
of vanillin related metabolites. Various aspects viz. polyamines, MJ and down stream process methodologies have been studied to enhance the metabolite production. It was concluded that hairy roots can be used as a
potential system for the production of betalaines. The organized nature and stable metabolite production makes them more amenable for bioreactor cultivation and
scale-up studies. Among all bioreactors studied, acoustic mist bioreactor performed well with high growth and metabolite production. There was no mass transfer
limitation observed in the acoustic mist bioreactor unlike others such as nutrient sprinkle and bubble column reactors. Osmolarity measurements proved reliable than
conductivity for indirect estimation of biomass when the hairy roots were cultivated in bioreactor systems. 180
The studies on product enhancement (polyamines and MJ) and on-line extraction of pigment (using CTAB) provided an insight for integrating unit operations and developing a process for continuous operation and higher production of
phytochemicals in bioreactor systems. It was found that the roots were still viable and showed normal growth even after CTAB treatment (0.002%) which would be an important observation to make this process a continuous one.
This study has shown the production of DOPA and dopamine, which are very important pharmaceutical compounds from hairy roots of B. vulgaris. Further studies with tyrosine feeding led to higher production of DOPA and dopamine. This study is of importance in understanding the biosynthetic pathway of betalaines derived from DOPA and dopamine. Use of oxygenase inhibitors such as caffeic acid and catechin formed the basis to envisage the enzymatic pathways in betalaine production. The higher production of DOPA and dopamine under the influence of caffeic acid and
catechin might be because of the inhibitory action of these chemicals on tyrosinase preventing further conversions.
 
Contributor Ravishankar, G.A
 
Date 2003-12
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1532/1/suresh.pdf
Suresh, B. (2003) Investigation on the Production of Food Ingredients and Biotransformation Using Hairy Root Cultures. PhD thesis, University of Mysore.