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Development of polyclonal ELISA for the N-methylcarbamate insecticide bendiocarb

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Relation http://ir.cftri.com/1605/
Food & Agri Immun-01-07
 
Title Development of polyclonal ELISA for the N-methylcarbamate insecticide bendiocarb
 
Creator Chetan, Anand
Akmal, Pasha
Swetha, M.D.
Putte, Gowda.
 
Subject 05 Insect/Pest Control
 
Description A polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) method was developed for the N-methylcarbamate insecticide bendiocarb (2,2-dimethyl-1,3-benzodioxol-4-yl methylcarbamate). Two novel haptens having dimethylbenzodioxyl and dimethylbenzofuranyl groups connected to oxyacetyl--aminobutanoic acid and oxyacetyl--alanine spacer arm respectively were synthesised. The first hapten was conjugated to carrier proteins to make antigens that were used to raise polyclonal antibodies in rabbits. The antibodies specifically recognised bendiocarb and its metabolite 2,2-dimethyl-1,3-benzodiox-4-ol with an IC50 value of 9 ppb (ng ml-1). The assay was standardised using the competitive ELISA format at 0.0625 µg antibody concentration and at 1/10k pesticide-HRP dilution. Matrix effect studies were carried out in four vegetable and cereal food samples. Matrix effect elimination in cabbage, cauliflower and rice was achieved by simple dilution of the extract. Five different approaches were attempted to achieve matrix clean up in paddy rice. C-18 column and gel permeation column chromatography (GPC) helped in the matrix removal. The spike and recovery studies for all the four food samples gave a recovery in the range of 75-95%, thus indicating the efficiency of the matrix elimination procedures developed.
 
Date 2007
 
Type Article
PeerReviewed
 
Identifier Chetan, Anand and Akmal, Pasha and Swetha, M.D. and Putte, Gowda. (2007) Development of polyclonal ELISA for the N-methylcarbamate insecticide bendiocarb. Food and Agricultural Immunology, 18 (1). pp. 39-51.