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Quantitative Studies on the Soluble Compartments of Light and Heavy Mitochondria from Rat Liver

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Relation http://ir.cftri.com/1614/
JBC-03-65
 
Title Quantitative Studies on the Soluble Compartments of Light
and Heavy Mitochondria from Rat Liver
 
Creator Wilhelm, R. Frisell
Madhav, V. Patwardhan
Cosmo, G. Mackenzie
 
Subject 07 Enzyme Biochemistry
 
Description Previous studies in our laboratories had shown that sarcosine dehydrogenase and dimethylglycine dehydrogenase are exclusively mitochondrial enzymes and that they can be liberated from mitochondria by sonic irradiation (2, 3). By employing the N-methyl-oxidizing enzymes as markers, the rates and extent of release of various mitochondrial components during sonic irradiation have been measured. Prom such analyses it has been possible to define more quantitatively the size and composition of the soluble compartment of the mitochondrion. It has been demonstrated also that sarcosine dehydrogenase, located
exclusively in the soluble compartment, provides a convenient marker for distinguishing “heavy” and “light” mitochondria.
 
Date 1965
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1614/1/J._Biol._Chem.%2C_Apr_1965_240_1829_-_1835.pdf
Wilhelm, R. Frisell and Madhav, V. Patwardhan and Cosmo, G. Mackenzie (1965) Quantitative Studies on the Soluble Compartments of Light and Heavy Mitochondria from Rat Liver. Journal of Biological Chemistry, 240 (4). pp. 1829-1835.