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The Complete Primary Structure of a Unique Mannose/Glucose-specific Lectin from Field Bean (Dolichos lab lab)*

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Relation http://ir.cftri.com/1619/
JBC-08-94
 
Title The Complete Primary Structure of a Unique
Mannose/Glucose-specific Lectin from Field Bean (Dolichos lab lab)*
 
Creator Lalitha, R. Gowda
Handanahal, S. Savithri
Rajagopal Rao, D.
 
Subject 29 Protein Chemistry
22 Legumes-Pulses
 
Description The complete amino acid sequence of two non-identi-
cal subunits of the glucose/mannose-specific lectin from
Dolichos lab lab (field bean) has been determined by
sequential Edman analyses of the intact subunits and
peptides derived by enzymatic and chemical cleavage.
Peptides were purified by reverse phase high perform-
ance liquid chromatography and ion pair chromatogra-
phy. The D. lab lab lectin is a glycoprotein having two
polypeptide chains of 132 and 106 amino acid residues.
The amino acid sequence of the D. lab lab lectin is com-
pared with the various lectins of the family Legumino-
sae. The D. lab lab lectin is the only species of the tribe
Phaseoleae that contains two nonidentical subunits of
almost equal size and that shows a specificity to glucose/
mannose. The lectin shows a greater homology to the
glucoselmannose-specific lectins, especially concanava-
lin A. The unique subunit architecture of the D. lab lab
lectin indicates the presence of new post-translational
cleavage sites.
 
Date 1994-07
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1619/1/J._Biol._Chem.%2C_Jul_1994_269_18789_-_18783.pdf
Lalitha, R. Gowda and Handanahal, S. Savithri and Rajagopal Rao, D. (1994) The Complete Primary Structure of a Unique Mannose/Glucose-specific Lectin from Field Bean (Dolichos lab lab)*. Journal of Biological Chemistry, 269 (29). pp. 18789-18793.