Record Details

Multiple forms of β-galactosidase from mango (Mangifera indica L Alphonso) fruit pulp

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/1771/
JSFA-16-05
 
Title Multiple forms of β-galactosidase from mango
(Mangifera indica L Alphonso) fruit pulp
 
Creator Prasanna, V.
Prabha, T. N.
Tharanathan, R. N.
 
Subject 06 Mango
14 Physical properties
28 Polysaccharide Chemistry
 
Description Mango (Mangifera indica L cv Alphonso) was found to contain three isoforms (I, II and III) of
β-galactosidase which, upon purification on Sephadex G-200, had relative abundances of 44, 38 and 18%,
respectively. The total specific activity increased from 20 to 727 µmol l−1 upon purification, representing a
∼36-fold increase with a recovery of 0.28UU−1. The optimal pH for activity and stability were in the ranges
3.6–4.3 and 4–6.2, respectively. The optimal temperature for β-galactosidase activity was between 42 and
47 ◦C with Tm in the range 45–51 ◦C. The Km for pNP-β-galactopyranoside was 0.98, 1.11 and 0.95mM,
and Vmax was 0.56, 0.53 and 0.35 µmol pNP min−1, respectively for isoforms I, II and III. Hg2+ caused
strong inhibition, whereas galacturonic acid, galactose, xylose, fucose and mannose slightly inhibited the
activity of β-galactosidase isoforms. The apparent molecular weights by GPC were 78, 58 and 91 kDa for
isoforms I, II and III, respectively. The ability of these isoforms to degrade the endogenous substrate
(arabinogalactan) possibly suggests a role in pectin dissolution during tissue softening/fruit ripening.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1771/1/J_Sci_Food_Agric_85_797-803_%282005%29.pdf
Prasanna, V. and Prabha, T. N. and Tharanathan, R. N. (2005) Multiple forms of β-galactosidase from mango (Mangifera indica L Alphonso) fruit pulp. Journal of the Science of Food and Agriculture, 85. pp. 797-803.