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Optimization and Interaction of Media Components in Ethanol Production Using Zymomonas mobilis by Response Surface Methodology

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Relation http://ir.cftri.com/1773/
JBB-01-99
 
Title Optimization and Interaction of Media Components in Ethanol
Production Using Zymomonas mobilis by Response
Surface Methodology
 
Creator Sreekumar, O.
Nagin, Chand.
Sidappa, C. Basappa
 
Subject 09 Food Microbiology
02 Bacteriology
 
Description Ethanol production using Zymomonas mobilis strains with medium optimized by response surface
methodology (RSM) was studied. A significant improvement in the ethanol yield (OSOg/g ethanol) was
demonstrated, resulting in very low residual sugar and fewer by-products. The five-factor and five-response full
factorial design have shown that glucose and yeast extract are the key media components that influence ethanol
production, followed by the inoculum concentration and ammonium sulfate, with phosphate showing little or
no effect. The composition (all in g/l) of the optimized medium was glucose 120.4, ammonium sulfate 0.96,
potassium dihydrogen phosphate 0.02, yeast extract 6.5, and magnesium sulfate 0.5. The optimum inoculum
concentration was log10 8.4 cells.
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/1773/1/JOURNAL_OF_BIOSCIENCE_AND_BIOENGINEERING.pdf
Sreekumar, O. and Nagin, Chand. and Sidappa, C. Basappa (1999) Optimization and Interaction of Media Components in Ethanol Production Using Zymomonas mobilis by Response Surface Methodology. Journal of Bioscience and Bioengineering, 88 (3). pp. 334-338.