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Enhancement of Growth and Coumarin Production in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L.cv.Lucknow local) under the Influence of Fungal Elicitors

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Relation http://ir.cftri.com/1774/
JBB-02-2000
 
Title Enhancement of Growth and Coumarin Production in Hairy Root
Cultures of Witloof Chicory (Cichorium intybus L.cv.Lucknow
local) under the Influence of Fungal Elicitors
 
Creator Harsh Pal, Bais
Sudha, Govindaswamy
Ravishankar, G. A.
 
Subject 10 Plants
05 Tissue Culture
 
Description Studies were conducted to determine the possible production of coumarins in hairy root cultures of CWo-
rium intybus L.cv.Lucknow local under the influence of microbial agents. pytlrium aphanidtwnatum and
Phytopthora parasitica var. nicotzizna were c&wed and their mycelia and medium titrate were used for the
elicitation of coumarin production. The media titrate of P. parasitica at 1.0% v/v added to the MS basai
medium led to the maximum growth of hairy roots of C. intybus, which was 1.57-fold higher than the untreated
control on the 28th day of culture, along with maximum escuUn and escuIelin yields which were 4.06- and 3.71-
fold higher than the control on the 28th day. The yields of es&in and esculetjll in hairy root cultmw of chicory
strongly correlated with growth. To check the effect of these fungal elicitors on endogenous polyamine
metabolism, titers of totaI endogenous polyamines were analyzed. It was confirmed that the media filtrate of P.
parasitica at 1.0% v/v concentration resulted in maximum accumulation of totai endogenous polyamines,
wherein endogenous spermine titers were found to be maximum as compared to endogenous spermidine and
putrescine titers on the 28th day. Total endogenous spermine in the a~ of 1.0% MF v/v P. paras&a was 1.3-
fold higher than that of the co&o1 on the 28th day of cuiture. Maximum growth index with greater length of
primary root (17.61 f 0.18 cm) and greater number of secondary and tertiary roots was recorded for the medium
filtrate of P. parasitica at 1.0% v/v concentration. This study also provided an insight into the morphologicai
changes in terms of branching patterns, occurring in roots under the influence of these fungal elidtors.
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1774/1/JOURNAL_OF_BIOSCIENCE_AND_BIOENGINEERING_90_%282000%29_648-653.pdf
Harsh Pal, Bais and Sudha, Govindaswamy and Ravishankar, G. A. (2000) Enhancement of Growth and Coumarin Production in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L.cv.Lucknow local) under the Influence of Fungal Elicitors. Journal of Bioscience and Bioengineering, 90 (6). pp. 648-653.