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Efficient amelioration of carbon tetrachloride induced toxicity in isolated rat hepatocytes by Syzygium cumini Skeels extract.

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Relation http://ir.cftri.com/1781/
Tox-01-08
 
Title Efficient amelioration of carbon tetrachloride induced toxicity in isolated rat hepatocytes by Syzygium cumini Skeels extract.
 
Creator Jyothi M, Veigas
Richa, Shrivastava
Bhagyalakshmi, N.
 
Subject 24 Fruits
32 Antioxidants
 
Description Syzygiumcumini, Indian black plum or Java plum, is a rich source for anthocyanins (230mg/100g DW) showing high antioxidant activity in vitro. In the following study it is further demonstrated that S. cumini peel extract rich in anthocyanins (SCA) offers considerable protection against carbon tetrachloride (CCl(4))-induced damage in rat hepatocytes. SCA itself being non-toxic to primary rat hepatocytes at concentrations ranging from 50 to 500ppm, was found to suppress CCl(4)-induced LDH leakage by 54% at 50ppm, thereby improving the cell viability by 39%. The SCA significantly reversed the CCl(4) induced changes in cellular glutathione (GSH) level, lipid peroxidation and activity of the antioxidant enzyme glutathione peroxidase. Exposure of hepatocytes to SCA after CCl(4) treatment was found to elevate GSH and GPx activities by 2-folds, whereas the activities of catalase and superoxide dismutase were not significantly affected. The fruit pulp extract (SPE) was less effective in offering protection to rat hepatocytes, particularly in terms of total GSH content and a consequent increase in lipid peroxidation although the higher GPx activity suggests the probable involvement of GSH as a substrate for GPx. These observations suggest that the fruit peel extract of S. cumini, is largely responsible for the reversal of CCl(4)-induced oxidative damage in rat hepatocytes. Both peel and pulp extract appear to offer protection to rat hepatocytes through GPx along with other biological pathways independent of catalase and superoxide dismutase.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1781/1/Toxicology_in_Vitro%2C_Volume_22%2C_Issue_6%2C_September_2008%2C_Pages_1440-1446.pdf
Jyothi M, Veigas and Richa, Shrivastava and Bhagyalakshmi, N. (2008) Efficient amelioration of carbon tetrachloride induced toxicity in isolated rat hepatocytes by Syzygium cumini Skeels extract. Toxicology In vitro, 22. pp. 1440-1446. ISSN 0887-2333 (Submitted)