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Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1783/
BRT-01-08
 
Title Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants.
 
Creator Sandesh Kamath, B.
Vidhyavathi, R.
Sarada, R.
Ravishankar, G. A.
 
Subject 01 Algae
14 Carotenoid Chemistry
 
Description Growing culture of green alga Haematococcus was exposed to mutagens such as UV, ethyl methane sulphonate (EMS) and 1-methyl 3-nitro 1-nitrosoguanidine (NTG), and further screened over herbicide - glufosinate. The survival rate of cells decreased with increasing concentration of mutagens and herbicides. The mutants exhibited 23-59% increase in total carotenoid and astaxanthin contents. The NTG treated glufosinate resistant mutant showed increased (2.2% to 3.8% w/w) astaxanthin content. The transcript levels of phytoene synthase, phytoene desaturase, lycopene cyclase, beta-carotene ketolase and beta -carotene hydroxylase enzymes in the mutant cultures were found to be 13-18, 14-17, 3, 3-22 and 6-20 fold higher respectively compared to wild type. The mutant obtained by UV irradiation showed highest lycopene cyclase activity (458 nmole beta -carotene formed/mg protein/h) followed by NTG mutant (315 nmole beta -carotene formed/mg protein/h) when compared to that of parent strain (105 nmole beta -carotene formed/mg protein/h). Expression analysis of carotenoid biosynthetic genes in the mutants exhibited increase in transcript levels compared to wild type.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1783/1/Bioresource_Technology%2C_Volume_99%2C_Issue_18%2C_December_2008%2C_Pages_8667-8673.pdf
Sandesh Kamath, B. and Vidhyavathi, R. and Sarada, R. and Ravishankar, G. A. (2008) Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants. Bioresource Technology, 99. pp. 8667-8673. ISSN 0960-8524