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Molecular Understanding of Copper and Iron Interaction with alpha-Synuclein by Fluorescence Analysis.

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Relation http://ir.cftri.com/1784/
JMN-01-08
 
Title Molecular Understanding of Copper and Iron Interaction with alpha-Synuclein by Fluorescence Analysis.
 
Creator Ms., Bharathi
Rao, K.S.J.
 
Subject 01 Medical sciences
 
Description alpha-Synuclein aggregation is a hallmark pathological feature in Parkinson's disease (PD). The conversion of alpha-synuclein from a soluble monomer to an insoluble fibril may underlie the neurodegeneration associated with PD. Redox-active metal ions such as iron (Fe) and copper (Cu) are known to enhance alpha-synuclein fibrillogenesis. In the present investigation, we analyzed the binding efficiency of Cu and Fe to alpha-synuclein by fluorescence studies. It is interesting to note that Cu and Fe showed differential binding pattern toward alpha-synuclein (wild type and A30P, A53T, and E46K mutant forms) as revealed by intrinsic tyrosine fluorescence, thioflavin-T fluorescence, 1-anilino-8-naphthalenesulfonate-binding studies, and scatchard plot analysis. The experimental data might prove useful in understanding the hierarchy of metals binding to alpha-synuclein and its role in neurodegeneration.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1784/1/Journal_of_Molecular_Neuroscience__2008_35_273-.pdf
Ms., Bharathi and Rao, K.S.J. (2008) Molecular Understanding of Copper and Iron Interaction with alpha-Synuclein by Fluorescence Analysis. Journal of Molecular Neuroscience, 35. pp. 273-281. ISSN 0895-8696