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Corncob-induced endo-1,4-beta-d-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan.

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Relation http://ir.cftri.com/1785/
JOAFC-28-08
 
Title Corncob-induced endo-1,4-beta-d-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan.
 
Creator Ayyappan, Appukuttan Aachary
Prapulla, S. G.
 
Subject 04 Fungi
 
Description Eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan (OSX) to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (XOS) production. Aspergillus oryzae MTCC 5154, Aspergillus flavus , Aspergillus niger , and Aspergillus ochraceus showed significant titers of endoxylanases, which were further used for the production of XOS from birch wood xylan (BWX). A. oryzae produced 89.5 +/- 1.13% XOS in the hydrolysate at 24 h of reaction. The effect of OSX, BWX, and raw corncob on the induction of endoxylanase in A. oryzae was studied, and the xylanase activity was maximum at 96 h of cultivation in 3% corncob containing medium. XOS produced at 36 h of reaction was 5.87 +/- 0.53 mg/mL (12 +/- 2% xylose, 48 +/- 2.43% xylobiose, and 40 +/- 3.6% higher oligomers) from 1% BWX . HPLC/refractive index detection and ESI/MS analysis of fractions obtained by GPC corresponded to neutral and 4- O-methyl-alpha- d-glucuronic acid substituted acidic oligosaccharides. The major fraction, beta- d-xylopyranosyl-(1-->4)- d-xylanopyranose was characterized using (13)C NMR.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1785/1/J._Agric._Food_Chem._2008%2C_56%2C_3981%E2%80%933988.pdf
Ayyappan, Appukuttan Aachary and Prapulla, S. G. (2008) Corncob-induced endo-1,4-beta-d-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan. Journal of Agricultural and Food Chemistry, 56 (11). pp. 3981-3988. ISSN 0021-8561