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Fractionation and purification of the phycobiliproteins from Spirulina platensis.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1810/
BRT-04-09
 
Title Fractionation and purification of the phycobiliproteins from Spirulina platensis.
 
Creator Patil, Ganapathi
Chethana, S.
Madhusudhan, M. C.
Raghavarao, K. S. M. S.
 
Subject 01 Algae
03 Proteins
 
Description C-Phycocyanin and allophycocyanin of Spirulina platensis are fractionated and purified using a non-chromatographic method namely, aqueous two phase extraction for the first time. Optimized process parameters of aqueous two phase extraction (PEG 4000/potassium phosphate of tie line length 18.64% with a phase volume ratio 1.45) resulted in pure C-phycocyanin and allophycocyanin with a purity of 3.23 and 0.74, respectively, in a single extraction. Multiple extractions (two) improved the purity of C-phycocyanin from 3.23 to 4.02. Integration of aqueous two phase extraction with membrane process not only facilitated the separation of phase forming components from the products and also increased the purity of allophycocyanin from 0.74 to 1.5.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1810/1/Bioresource_Technology%2C_Volume_99%2C_Issue_15%2C_October_2008%2C_Pages_7393-7396.pdf
Patil, Ganapathi and Chethana, S. and Madhusudhan, M. C. and Raghavarao, K. S. M. S. (2008) Fractionation and purification of the phycobiliproteins from Spirulina platensis. Bioresource Technology, 99 (15). pp. 7393-6. ISSN 0960-8524