Record Details

Liquid–liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/1813/
 
Title Liquid–liquid extraction of bromelain and polyphenol
oxidase using aqueous two-phase system
 
Creator Ravindra Babu, B.
Rastogi, N. K.
Raghavarao, K.S.M.S.
 
Subject 05 Enzymes
24 Fruits
07 Food Engineering
 
Description Aqueous two-phase extraction is employed for the first time for separation and purification of mixture of enzymes (bromelain and polyphenol
oxidase) from the pineapple (Ananas comosus L. Merr.). Influence of several parameters such as phase forming polymer molecular weight
(polyethylene glycol 1500–20,000), concentration of phase forming components (polyethylene glycol, 12–18%, w/w, and potassium phosphate,
14–20%, w/w) and system pH (6.0–9.0) on differential partitioning of bromelain and polyphenol oxidase required for the purification is studied.
Bromelain preferentially partitioned to the top (polyethylene glycol) phase, while polyphenol oxidase to the bottom (potassium phosphate) phase.
Partition coefficient of both the enzymes decreased with an increase in molecular weight of polyethylene glycol. Phase composition of the system
was found to have significant effect on enzyme partitioning and degree of purification. The polyethylene glycol/potassium phosphate system
(comprising of 18% PEG 1500 and 14% phosphate) resulted in about 228% activity recovery and 4.0-fold increase in purity in case of bromelain
and about 90% activity recovery and 2.7-fold increase in purity of polyphenol oxidase.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1813/1/Chemical%20Engineering%20and%20Processing%20Process%20Intensification%2C%20Volume%2047%2C%20Issue%201%2C%20January%202008%2C%20Pages%2083-89.pdf
Ravindra Babu, B. and Rastogi, N. K. and Raghavarao, K.S.M.S. (2008) Liquid–liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system. Chemical Engineering and Processing: Process Intensification, 47. pp. 83-89. ISSN 0255-2701