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Inhibition of lipoxygenase by soy isoflavones: evidence of isoflavones as redox inhibitors.

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Relation http://ir.cftri.com/1876/
ABB-01-07
 
Title Inhibition of lipoxygenase by soy isoflavones: evidence of isoflavones as redox inhibitors.
 
Creator Mahesha, H. G.
Sridevi Annapurna, Singh
Appu Rao, A. G.
 
Subject 05 Soya bean
16 Enzyme Chemistry
 
Description Hydroperoxides, the products of lipoxygenase mediated pathways, play a major role in the manifestation of chronic inflammatory diseases. Soy isoflavones act as antioxidants due to their ability to scavenge free radicals. Isoflavones inhibit the activity of soy lipoxygenase-1 and 5-lipoxygenase, from human polymorph nuclear lymphocyte in a concentration dependent manner. Spectroscopic and enzyme kinetic measurements have helped to understand the nature and mechanism of inhibition. Genistein is the most effective inhibitor of soy lipoxygenase 1 and 5-lipoxygenase with IC(50) values of 107 and 125 microM, respectively. Genistein and daidzein are noncompetitive inhibitors of soy lipoxygenase 1 with inhibition constants, K(i), of 60 and 80 microM, respectively. Electron paramagnetic resonance and spectroscopic studies confirm that isoflavones reduce active state iron to ferrous state and prevent the activation of the resting enzyme. A model for the inhibition of lipoxygenase by isoflavones is suggested.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1876/1/Archives_of_Biochemistry_and_Biophysics_461%282%29_2007_176-.pdf
Mahesha, H. G. and Sridevi Annapurna, Singh and Appu Rao, A. G. (2007) Inhibition of lipoxygenase by soy isoflavones: evidence of isoflavones as redox inhibitors. Archives of Biochemistry and Biophysics, 461 (2). pp. 176-85. ISSN 0003-9861