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Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1900/
CR-01-07
 
Title Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage.
 
Creator Harish Prashanth, K. V.
Shylaja, M. Dharmesh
Jagannatha Rao, K. S.
Tharanathan, R. N.
 
Subject 11 Food Biochemistry
 
Description Low molecular weight chitosan (LMWC) and chitooligosaccharides (COs), obtained by persulfate-induced depolymerization of chitosan showed scavenging of OH. and O2.- radicals and offered protection against calf thymus DNA damage. Over 85% inhibition of free radicals and DNA protection were observed. LMWC (0.05 micromol) showed a strong inhibitory activity compared to COs (3.6 micromol). Further, LMWC showed calf thymus DNA condensation reversibly giving stability, as evident from CD, TEM and melting curves (Tm). A fluorescence study suggests the binding of LMWC in the minor groove, forming H-bonds to the backbone phosphates without distorting the double helix structure.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1900/1/Carbohydrate_Research_342%282%29_2007_190-195.pdf
Harish Prashanth, K. V. and Shylaja, M. Dharmesh and Jagannatha Rao, K. S. and Tharanathan, R. N. (2007) Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage. Carbohydrate research, 342 (2). pp. 190-5. ISSN 0008-6215