The 53-kDa proteolytic product of precursor starch-hydrolyzing enzyme of Aspergillus niger has Taka-amylase-like activity.
IR@CSIR-CFTRI
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Relation |
http://ir.cftri.com/1902/
AMB-04-07 |
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Title |
The 53-kDa proteolytic product of precursor starch-hydrolyzing enzyme of Aspergillus niger has Taka-amylase-like activity.
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Creator |
Ravi Kumar, K
Venkatesh, K. S. Umesh Kumar, S. |
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Subject |
05 Enzymes
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Description |
The 53-kDa amylase secreted by Aspergillus niger due to proteolytic processing of the precursor starch-hydrolyzing enzyme was resistant to acarbose, a potent alpha-glucosidase inhibitor. The enzyme production was induced when A. niger was grown in starch medium containing the inhibitor. Antibodies against the precursor enzyme cross-reacted with the 54-kDa Taka-amylase protein of A. oryzae. It resembled Taka-amylase in most of its properties and also hydrolyzed starch to maltose of alpha-anomeric configuration. However, it did not degrade maltotriose formed during the reaction and was not inhibited by zinc ions.
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Date |
2007
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Identifier |
http://ir.cftri.com/1902/1/Appl_Microbiol_Biotechnol_%282007%29_74_1011%E2%80%931015.pdf
Ravi Kumar, K and Venkatesh, K. S. and Umesh Kumar, S. (2007) The 53-kDa proteolytic product of precursor starch-hydrolyzing enzyme of Aspergillus niger has Taka-amylase-like activity. Applied Microbiology and Biotechnology, 74 (5). pp. 1011-5. ISSN 0175-7598 |
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