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Isolation and characterization of dihydromonacolin-MV from Monascus purpureus for antioxidant properties.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1907/
AMB-03-07
 
Title Isolation and characterization of dihydromonacolin-MV from Monascus purpureus for antioxidant properties.
 
Creator Dhale, M. A.
Divakar, S.
Umesh Kumar, S.
Vijayalakshmi, G.
 
Subject 01 Rice
04 Fermentation Technology
32 Antioxidants
 
Description The methanolic extract of Monascus purpureus cultivated by solid-state fermentation on rice showed strong 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and better yield as compared to other polarity based extracted fractions. It was selected for further purification of the antioxidant. The activity-guided repeated fractionation of methanolic extract on a silica gel column chromatography yielded a compound that exhibited strong antioxidant activity. Based on the spectroscopic analysis by UV, IR, 1H NMR, 13C NMR, 2D-HSQCT NMR, and MS, the antioxidant isolated was elucidated as a derivative of dihydromonacolin-K, where the ester group is 2-methyl propionate, designated as dihydromonacolin-MV. The DPPH radical was significantly scavenged by the dihydromonacolin-MV (IC50 20+/-1 microg ml-1). The dihydromonacolin-MV showed strong inhibition of lipid peroxidation in a liposome model with an IC50 value of 5.71+/-0.38 microg ml-1 and superoxide radical scavenging activity with an IC50 value of 163.97+/-2.68 microg ml-1.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1907/1/Appl_Microbiol_Biotechnol_%282007%29_73_1197%E2%80%931202.pdf
Dhale, M. A. and Divakar, S. and Umesh Kumar, S. and Vijayalakshmi, G. (2007) Isolation and characterization of dihydromonacolin-MV from Monascus purpureus for antioxidant properties. Applied Microbiology and Biotechnology, 73 (5). pp. 1197-202. ISSN 0175-7598