ICAR Research Data Repository for Knowledge Management
Method to obtain C-phycocyanin of high purity.
IR@CSIR-CFTRI
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| Relation |
http://ir.cftri.com/1932/
JC-01-06 |
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| Title |
Method to obtain C-phycocyanin of high purity.
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| Creator |
Patil, Ganapathi
Chethana, S. Sridevi, A. S. Raghavarao, K. S. M. S. |
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| Subject |
01 Algae
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| Description |
A new approach is made for the purification of C-phycocyanin (C-PC), which is simple and more efficient than existing methods. The proposed method involves two steps: aqueous two phase extraction and ion-exchange chromatography. Crude extract of C-phycocyanin, of purity 1.18, obtained from Spirulina platensis is subjected to aqueous two phase extraction. C-phycocyanin obtained from this process showed a purity of 5.22, which is higher than the reported value till date. In order to explore the possibility of further purification, C-phycocyanin is subjected to ion-exchange chromatography and found that the purity increased from 5.22 to 6.69. The fluorescence, intactness of structure and purity of C-phycocyanin are confirmed using spectrofluorometry, circular dichroism spectra and sodium dodecyl sulfate-polyacrylamide gel, respectively.
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/1932/1/Journal_of_Chromatography_A_1127%281-2%29_2006_76-81.pdf
Patil, Ganapathi and Chethana, S. and Sridevi, A. S. and Raghavarao, K. S. M. S. (2006) Method to obtain C-phycocyanin of high purity. Journal of chromatography. A, 1127 (1-2). pp. 76-81. ISSN 0021-9673 |
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