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Influence of 8-methyl-nonenoic acid on capsaicin biosynthesis in in vivo and in vitro cell cultures of Capsicum spp.

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Title Influence of 8-methyl-nonenoic acid on capsaicin biosynthesis in in vivo and in vitro cell cultures of Capsicum spp.
 
Creator Narasimha Prasad, B. C.
Gururaj, Harishchandra Bhaskar
Vinod, Kumar
Giridhar, Parvatam
Parimalan, Rangan
Sharma, Ashwani
Ravishankar, G. A.
 
Subject 23 Vegetables
01 Plant Cell
05 Tissue Culture
08 Food Chemistry
 
Description Capsaicin is a bioactive molecule synthesized by enzymatic (putative capsaicin synthase) condensation of vanillylamine, a phenyl propanoid intermediate with 8-methyl-nonenoic acid, a fatty acid derivative from leucine/valine pathway. Analysis of levels of 8-methyl-nonenoic acid and phenyl propanoid intermediates in high, medium, and low pungent Capsicum genotypes revealed that the 8-methyl-nonenoic acid pool plays a crucial role in determining the efficacy of capsaicin levels. Cerulenin-mediated inhibition of 8-methyl-nonenoic acid synthesis decreased the capsaicin biosynthesis in Capsicum cell suspension cultures. Similarly amino oxy acetate inhibited vanillylamine synthesis but failed to reduce capsaicin production. The mRNA transcript analysis of keto acyl synthase (KAS), a crucial enzyme involved in 8-methyl-nonenoic acid and an amino transferase (AMT), involved in vanillylamine biosynthesis was studied. The mRNA transcript analysis revealed the progressive developmental expression of KAS gene in the placenta during the ontogeny of the fruit, whereas AMT transcripts levels did not show significant differences. Hence, the study demonstrates the influence of 8-methyl-nonenoic acid and its possible regulatory role in capsaicin biosynthesis in Capsicum spp.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/1941/1/JAgricFdChem541854-1859.pdf
Narasimha Prasad, B. C. and Gururaj, Harishchandra Bhaskar and Vinod, Kumar and Giridhar, Parvatam and Parimalan, Rangan and Sharma, Ashwani and Ravishankar, G. A. (2006) Influence of 8-methyl-nonenoic acid on capsaicin biosynthesis in in vivo and in vitro cell cultures of Capsicum spp. Journal of Agricultural and Food Chemistry, 54. pp. 1854-1859. ISSN 0021-8561