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A comparative study on permeabilization treatments for in situ determination of phytase of Rhodotorula gracilis

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Relation http://ir.cftri.com/1975/
LAM-01-98
 
Title A comparative study on permeabilization treatments for in
situ determination of phytase of Rhodotorula gracilis
 
Creator Bindu, S.
Somashekar, D.
Joseph, R.
 
Subject 19 Yeast
16 Enzyme Chemistry
 
Description Rhodotorula gracilis, a red yeast, was
shown to produce phytase and the cultural conditions were standardized for
phytase production. Permeabilization treatments using detergents, organic solvents,
enzymes and physical methods were performed and compared by measuring the phytase
activity of whole cells. None of the permeabilization methods except the freeze-thaw
method enhanced phytase activity. The enzyme activity of a yeast suspension
subjected to 15 cycles of freezing and thawing was 77·26 U g�1 cells. Phytate in feeds
is an anti-nutritional factor which complexes with many minerals, rendering it
unavailable to monogastric animals. Rhodotorula gracilis subjected to freeze-thawing can
be used as a source of phytase in feeds to enhance phosphorus availability.
 
Date 1998
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1975/1/Letters_in_Applied_Microbiology_1998%2C_27%2C_336%E2%80%93340.pdf
Bindu, S. and Somashekar, D. and Joseph, R. (1998) A comparative study on permeabilization treatments for in situ determination of phytase of Rhodotorula gracilis. Letters in Applied Microbiology, 27. pp. 336-340.