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Chitooligosaccharides--preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity.

IR@CSIR-CFTRI

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CR-06-05
 
Title Chitooligosaccharides--preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity.
 
Creator Kittur, Farooqahamed S
Vishu Kumar, Acharya B
Varadaraj, M. C.
Tharanathan, R. N.
 
Subject 28 Meat, Fish & Poultry
28 Polysaccharide Chemistry
 
Description An isozyme of pectinase from Aspergillus niger with polygalacturonase activity caused chitosanolysis at pH 3.5, resulting in low-molecular weight chitosan (86%), chitooligosaccharides (COs, 4.8%) and monomers (2.2%). HPLC showed the presence of COs with DP ranging from 2 to 6. Charcoal-Celite chromatography and re-N-acetylation of the COs followed by CD, IR, MALDI-TOF-MS and FAB-MS analyses revealed an abundance of chitobiose, chitotriose and chitotetraose. The COs-monomeric mixture showed a bactericidal effect towards Bacillus cereus and Escherichia coli more efficiently than native chitosan. Among the chitooligomers, the hexamer showed maximum antibacterial effect followed by the penta-, tetra-, tri- and dimers. Of the two monomers, only GlcN showed slight bacterial growth inhibition. SEM revealed bactericidal action patterns of COs-monomeric mixture towards B. cereus and E. coli.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1996/1/Carbohydrate_Research_340_%282005%29_1239-1245.pdf
Kittur, Farooqahamed S and Vishu Kumar, Acharya B and Varadaraj, M. C. and Tharanathan, R. N. (2005) Chitooligosaccharides--preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity. Carbohydrate research, 340 (6). pp. 1239-45. ISSN 0008-6215