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Scanning electron microscopy of chemically and enzymatically treated black gram (Phaseolus mungo) and ragi (Eleusine coracana) starch granules

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STA-04-88
 
Title Scanning electron microscopy of chemically and enzymatically treated black gram (Phaseolus mungo) and ragi (Eleusine coracana) starch granules
 
Creator Tharanathan, R. N.
Ramadas Bhat, U.
 
Subject 21 Cereals
30 Starch Chemistry
22 Legumes-Pulses
 
Description Amylolysis of chemically-treated starch granules of black gram and ragi was followed colorimetrically and by scanning electron microscopy (SEM). % hydrolysis values indicated that oxidation with periodate resulted in considerable resistance, whereas urea treatment resulted in granule susceptibility towards in vitro amylolysis. The action of alpha-amylase and/or glucoamylase on periodate oxidized black gram starch showed on SEM enlargement of the granules and hollow craters, whereas similar treatment on oxidized ragi starch resulted in folded, distored and deformed granules. Treatment with 8M urea resulted in total loss of characteristic granular shape and formation of so-called amylopectin `sacs'. Synergistic action of alpha-amylase and glucoamylase on urea-treated starches virtually collapsed the granules, forming innumerable `flat' ribbon-like fragments.
 
Date 1988
 
Type Article
PeerReviewed
 
Identifier Tharanathan, R. N. and Ramadas Bhat, U. (1988) Scanning electron microscopy of chemically and enzymatically treated black gram (Phaseolus mungo) and ragi (Eleusine coracana) starch granules. Starch/Staerke, 40 (10). pp. 378-382. ISSN 0038-9056