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Staphylococcal accessory gene regulator (sar) as a signature gene to detect enterotoxigenic staphylococci.

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Title Staphylococcal accessory gene regulator (sar) as a signature gene to detect enterotoxigenic staphylococci.
 
Creator Padmapriya, B. P.
Ramesh, A.
Chandrashekar, A.
Varadaraj, M. C.
 
Subject 09 Food Microbiology
 
Description AIMS: To evaluate the use of a staphylococcal accessory gene regulator (sar) as a means of detecting enterotoxigenic staphylococci. METHODS AND RESULTS: SarA gene-specific primers were designed and applied in PCR, which resulted in the detection of 49 sar-positive isolates from a total of 67 natural food isolates of staphylococci. Colony hybridization using PCR-generated Digoxigenin (DIG)-labelled sarA probe tested in spiked samples of khoa (a traditional heat-concentrated milk product) comprising a mixed microflora ensured the specificity of the probe. Validation experiments with the commercial samples of khoa also demonstrated the specificity of the probe. PCR characterization for enterotoxins A-D revealed the presence of at least one of the toxin-encoding genes in all the sarA-positive isolates tested. CONCLUSION: The study indicated that sarA gene could be an ideal marker gene either in colony hybridization or in PCR, for an effective detection of potentially enterotoxigenic strains of staphylococci in a food system. SIGNIFICANCE AND IMPACT OF THE STUDY: As an alternative to targeting the individual toxin genes, a regulatory gene responsible for controlling the synthesis of various virulence factors may be a suitable target gene for screening potentially toxigenic staphylococci in food system using nucleic acid-based methods.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2049/1/Journal_of_Applied_Microbiology_2003%2C_95%2C_974-981.pdf
Padmapriya, B. P. and Ramesh, A. and Chandrashekar, A. and Varadaraj, M. C. (2003) Staphylococcal accessory gene regulator (sar) as a signature gene to detect enterotoxigenic staphylococci. Journal of Applied Microbiology, 95 (5). pp. 974-81. ISSN 1364-5072