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Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti.

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Title Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti.
 
Creator Lakshman, Kshama
Shamala, Tumkur Ramachandriah
 
Subject 09 Food Microbiology
 
Description Strains of Rhizobium spp. isolated from leguminous plants and standard strains accumulated 27% to 57% polyhydroxyalkanoate (PHA) of their cell biomass. Among these cultures, one strain of Rhizobium meliloti synthesized 10-30% more PHA than others and contained 3% hydroxyvalerate (HV) when grown on sucrose as carbon substrate. The occurrence of hydroxybutyrate (HB) and HV was confirmed by GC and 1H NMR analysis. Treatment of the culture with 4'-N-piperidinobutyl-2-chlorophenoxazine resulted in a mutant which synthesized up to 69%, PHA of the cell biomass with an improved yield of 11 to 47% under different carbon and nitrogen ratios, compared to the parent strain.
 
Date 2003
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2060/1/Biotechnol_Letters_2003_25_2_115-119.pdf
Lakshman, Kshama and Shamala, Tumkur Ramachandriah (2003) Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti. Biotechnology Letters, 25 (2). pp. 115-119. ISSN 0141-5492