Record Details

Relationship Between the Level of Signaling Lymphocyte Activation Molecule mRNA and Replication of Peste-Des-Petits-Ruminants Virus in Peripheral Blood Mononuclear Cells of Host Animals

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Relationship Between the Level of Signaling Lymphocyte Activation Molecule mRNA and Replication of Peste-Des-Petits-Ruminants Virus in Peripheral Blood Mononuclear Cells of Host Animals
 
Creator Pawar, R.M.
Raj, G. Dhinakar
Balachandran, C.
 
Subject Peste-des-petits-ruminants virus
Signaling lymphocyte activation molecule
peripheral blood mononuclear cells
real-time PCR
 
Description Basal signaling lymphocyte activation molecule (SLAM) expression in the peripheral blood mononuclear cells (PBMCs) of cattle, buffalo, sheep, and goats was correlated with Peste-des-petits-ruminants virus (PPRV) replication assessed by real-time PCR and virus titers. PBMCs from goats had the highest level of SLAM mRNA followed by sheep, cattle, and buffalo. Different breeds of goats had different basal levels of SLAM mRNA. In the PBMCs of studied animals, basal SLAM expression had high correlation with their ability to replicate PPRV. After stimulation of PBMCs from different breeds of goats with concanavalin A (con A), the SLAM expression increased 4- to 16-fold, what resulted in a 1.7-to 3.8-fold increase of viral mRNA and the virus titer increased by 0.4-1.3 log units. These findings revealed that SLAM expression and PPRV replication are highly correlated and different levels of SLAM mRNA could influence the virus replication in different animals.
 
Date 2016-08-08T15:35:52Z
2016-08-08T15:35:52Z
2008
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/71608
 
Language en
 
Format application/pdf
 
Publisher Act Virological