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High yield production of folates from soybean callus cultures in response to elicitors.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/13538/
https://doi.org/10.1007/s13205-018-1101-x
 
Title High yield production of folates from soybean callus cultures
in response to elicitors.
 
Creator Akitha Devi, M. K.
Sravan Kumar, Sandopu
Giridhar, P.
 
Subject 01 Dietary Fiber
05 Soya bean
 
Description Soybean (Indian variety, JS 335) callus growth and its folate content was sustained on Murashige and Skoog (MS) medium
supplemented with optimized levels of auxins and cytokinins. Callus cultures exhibited moderate production of total folate.
Callus growth was stimulated with highest biomass content of 21.3 g/L DW (24 days culture) on medium supplemented
with 1.5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.1 mg/L Kinetin (Kn). Total folate production was enhanced by
an increase in indole-3-acetic acid (IAA) from 0.5 to 1.0 mg/L and decreased thereafter up to 1.5 mg/L. 2,4-D at 1.5 mg/L
repressed the callus growth and also folate production. A concentration of 1.0 mg/L IAA and 0.2 mg/L Kn elicited folate
production to a maximum total folate content of 323.82 μg/100 g DW. The results indicate that optimized medium for callus
suspension cultures conceivably are applied for scale-up studies in bioreactors.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13538/1/3%20Biotech%20%282018%29%20880.pdf
Akitha Devi, M. K. and Sravan Kumar, Sandopu and Giridhar, P. (2018) High yield production of folates from soybean callus cultures in response to elicitors. 3 Biotech, 8. pp. 1-10.