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Lutein downregulates retinal vascular endothelial growth factor possibly via hypoxia inducible factor 1 alpha and X-box binding protein 1 expression in streptozotocin induced diabetic rats.

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Relation http://ir.cftri.com/13580/
http://dx.doi.org/10.1016/j.jff.2017.01.023
 
Title Lutein downregulates retinal vascular endothelial growth factor possibly
via hypoxia inducible factor 1 alpha and X-box binding protein 1
expression in streptozotocin induced diabetic rats.
 
Creator Sharavana, Gurunathan
Baskaran, V.
 
Subject 14 Carotenoid Chemistry
04 Diabetes Mellitus
 
Description Lutein, a retinal-specific carotenoid, was assessed for angioprotective efficacy via hypoxia inducible factor
(HIF1a) and X-box binding protein 1 (XBP1) pathway in diabetic retina. Streptozotocin-induced diabetic
rats were orally gavaged with or without lutein (39 nmol/day) for 8 weeks. Lutein mediated regulation of
vascular endothelial growth factor (VEGF) and superoxide dismutase 2 (SOD2) was analyzed using qPCR
and immunoblotting. VEGF transcription factors, Hif1a and XBP1, in retina of streptozotocin induced diabetic
rats were assessed using qPCR. Retinal protein carbonyl and lipid peroxide levels were quantified
and transverse section of retina was stained for histopathology. Results indicate that lutein prevented
the upregulation of VEGF and downregulation of SOD2 in retina of diabetic rats and also prevented abnormalities
in ganglion cell, inner and outer nuclear layer in diabetic retina. This study suggests the anti
VEGF property of lutein is perhaps via decrease in the mRNA expression of Hif1a and Xbp1.
 
Publisher Elsevier
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13580/1/Journal%20of%20Functional%20Foods%2031%20%282017%29%2097%E2%80%93103.pdf
Sharavana, Gurunathan and Baskaran, V. (2017) Lutein downregulates retinal vascular endothelial growth factor possibly via hypoxia inducible factor 1 alpha and X-box binding protein 1 expression in streptozotocin induced diabetic rats. Journal of Functional Foods, 31. pp. 97-103. ISSN 1756-4646