Hypolipidemic and Antioxidant Properties of Oryzanol Concentrate in Reducing Diabetic Nephropathy via SREBP1 Downregulation Rather than β-Oxidation.
IR@CSIR-CFTRI
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Relation |
http://ir.cftri.com/13587/
https://dx.doi.org/10.1002/mnfr.201700511 |
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Title |
Hypolipidemic and Antioxidant Properties of Oryzanol Concentrate in Reducing Diabetic Nephropathy via SREBP1 Downregulation Rather than β-Oxidation. |
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Creator |
Bhaskaragoud, G.
Geetha, V. Sharanappa, T. Mohan Kumar, A. S. Hemakumar, C. Suresh Kumar, G. |
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Subject |
11 Lipid Biochemistry
04 Diabetes Mellitus |
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Description |
Scope: Diabetic nephropathy (DN) is a micro-vascular complication of chronic diabetes. Sterol regulatory element binding protein1 (SREBP1) participation in the development of DN is reported. Oryzanol concentrate (OC) at 0.1% and 0.3% is tested for its antioxidant and hypolipidemic effects. The aim of the work is to study the involvement of OC in the amelioration of DN in STZ-induced diabetic animal model. Methods and results: Animals were grouped into starch, high-fat, and OC-treated control/diabetic groups (SFC/SFD, HFC/HFD, OFC/OFD). The markers of DN, increased glomerular filtration rate and kidney weight, were evident in HFD and reduced in OFD group by �1.09 and �1.3 fold, respectively. The amelioration of defensive antioxidant enzyme activities and lipid peroxidation, expressions of lipid-associated biomolecules (SREBP1 and FAS) were also observed. HFD showed increased ECM accumulation of glycoproteins, particularly Type IV collagen, fibronectin. SREBP1-associated gene transforming growth factor-β (TGF-β) was reduced on treatment (OFD � 1.3 fold) as to HFD (�2.7 fold). Conclusion: Oryzanol concentrate, having hypolipidemic and antioxidant properties, also downregulated the lipid biosynthesis through reduced SREBP1–TGF-β interactions (EMSA) and could effectively ameliorate DN. Gene (ACC2, Cpt1, and ACOX) expression studies showed that β-oxidation was not involved in reducing DN. |
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Date |
2018
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Type |
Article
PeerReviewed |
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Format |
pdf
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Language |
en
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Identifier |
http://ir.cftri.com/13587/1/Molecular_Nutrition_%2526_Food_Research.pdf
Bhaskaragoud, G. and Geetha, V. and Sharanappa, T. and Mohan Kumar, A. S. and Hemakumar, C. and Suresh Kumar, G. (2018) Hypolipidemic and Antioxidant Properties of Oryzanol Concentrate in Reducing Diabetic Nephropathy via SREBP1 Downregulation Rather than β-Oxidation. Molecular Nutrition and Food Research, 62. pp. 101-11. ISSN 1613-4125 |
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