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Subinhibitory concentrations of gentamicin trigger expression of aac(6´)Ie-aph(2˝)Ia, chaperones and biofilm-related genes in Lactobacillus plantarum MCC3011.

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Relation http://ir.cftri.com/13593/
https://dx.doi.org/10.1016/j.resmic.2017.06.002
 
Title Subinhibitory concentrations of gentamicin trigger expression of aac(6´)Ie-aph(2˝)Ia,
chaperones and biofilm-related genes in Lactobacillus plantarum MCC3011.
 
Creator Jaimee, George
Prakash, M. Halami
 
Subject 02 Bacteriology
 
Description The study aimed to analyze the effects of sub-inhibitory concentrations of gentamicin on the expressions of high level aminoglycoside resistant (HLAR) bifunctional aac(6')Ie-aph(2″)Ia, biofilm and chaperone genes in Lactobacillus plantarum. The analysis of the biofilm formation in five isolates obtained from chicken sausages indicated their role in exhibiting phenotypic resistance based on the varied MIC values despite carrying the bifunctional gene. The biofilm formation significantly increased when L. plantarum MCC 3011 was grown in sub-inhibitory concentrations of gentamicin (4 μg/ml), kanamycin (8 μg/ml) and streptomycin (2 μg/ml). Thirty day gentamicin selection increased minimum inhibitory concentration (MIC) values from 4 to 64 and 2 to 256 fold for gentamicin and kanamycin, respectively when compared to the parental cultures. Expression studies revealed that constant exposure to gentamicin had induced chaperon [groEL] and the bifunctional gene, aac(6')Ie-aph(2″)Ia upto nine fold. Induction of groEL, groES and lamC genes in gentamicin (4 μg/ml) preincubated MCC 3011 indicated their significant role in aminoglycoside mediated response. Our study indicates that constant exposure to sub inhibitory concentrations of gentamicin allows L. plantarum to adapt against higher doses of aminoglycosides. This highlights the risks and food safety issues associated with the use of aminoglycosides in livestock and consumption of farm oriented fermented food products.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13593/1/Research%20in%20Microbiology%20%282017%29.pdf
Jaimee, George and Prakash, M. Halami (2017) Subinhibitory concentrations of gentamicin trigger expression of aac(6´)Ie-aph(2˝)Ia, chaperones and biofilm-related genes in Lactobacillus plantarum MCC3011. Research in Microbiology, 168 (8). pp. 722-731.