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Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.

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Relation http://ir.cftri.com/13817/
 
Title Quantification of Neurotransmitters from Intact
and Regenerating Planarians Using UHPLC-MS/SRM Method.
 
Creator Kannan, Rangiah
Palakodeti, Dasaradhi
 
Subject 06 Chromatography
Fish
 
Description Freshwater planarian species S. mediterranea is an emerging stem cell model because of its capability of
regenerating large portions of missing body parts. It is one of the best model systems available to address
the basic biological mechanisms in the regeneration processes. Absolute quantification of metabolites from
planarians is imperative to understand their role in the regeneration processes. Here we describe a stable
isotope dilution ultrahigh performance liquid chromatography/mass spectrometry/selected reaction
monitoring (UHPLC-MS/SRM) assay for a sensitive and quantitative assessment of neurotransmitters
(NTs) in planaria. We used this method for the simultaneous quantification of 16 NTs from both intact
and regenerating planarians.
 
Publisher Springer
 
Date 2018
 
Type Book Section
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13817/1/Methods%20in%20Molecular%20Biology%2C%20vol.%201774.pdf
Kannan, Rangiah and Palakodeti, Dasaradhi (2018) Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method. In: Planarian Regeneration: Methods and Protocols, Methods in Molecular Biology. Springer, pp. 555-570.