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Arabidopsis serine/threonine/ tyrosine protein kinase phosphorylates oil body proteins that regulate oil content in the seeds.

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Relation http://ir.cftri.com/13831/
https://dx.doi.org/10.1038/s41598-018-19311-3
 
Title Arabidopsis serine/threonine/
tyrosine protein kinase
phosphorylates oil body proteins
that regulate oil content in the
seeds.
 
Creator Iyappan, R.
Anitha, Vijayakumar
Ramya, V.
Ram, Rajasekharan
 
Subject 10 Plants
03 Proteins
19 Lipids-oils/fats
 
Description Protein phosphorylation is an important post-translational modification that can regulate the protein
function. The current knowledge on the phosphorylation status of plant oil body (OB) proteins is
inadequate. This present study identifies the distinct physiological substrates of Arabidopsis serine/
threonine/tyrosine protein kinase (STYK) and its role in seed oil accumulation; the role of Arabidopsis
OLE1, a major seed OB protein has also been elucidated. In vitro kinase assay followed by mass
spectrometry identifies residue that are phosphorylated by STYK. Further, co-expression of OLE1 and
STYK in yeast cells increases the cellular lipid levels and reduces the total lipid when OLE1 was replaced
with OLE1T166A. Moreover, in vivo experiments with OB isolated from wild-type and styk knock-out
lines show the ability of STYK to phosphorylate distinct OB proteins. OLE1T166A mutant and Arabidopsis
styk mutant demonstrate the significant reduction of its substrate phosphorylation. styk mutant line
significantly reduces the amount of total seed oil as compared to wild-type seeds. Together, our results
provide the evidences that Arabidopsis At2G24360 (STYK) is phosphorylating oil body proteins and the
phosphorylation regulates the oil content in Arabidopsis seeds.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13831/1/SCientifiC%20REPOrTS%20%20%282018%29%2081154.pdf
Iyappan, R. and Anitha, Vijayakumar and Ramya, V. and Ram, Rajasekharan (2018) Arabidopsis serine/threonine/ tyrosine protein kinase phosphorylates oil body proteins that regulate oil content in the seeds. Scientific Reports, 8 (1154). pp. 1-14.