Record Details

Low-dose doxorubicin with carotenoids selectively alters redox status and upregulates oxidative stress-mediated apoptosis in breast cancer cells.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/13838/
https://doi.org/10.1016/j.fct.2018.06.027
 
Title Low-dose doxorubicin with carotenoids selectively alters redox status and
upregulates oxidative stress-mediated apoptosis in breast cancer cells.
 
Creator Vijay, K.
Sowmya, P. R. Rao
Arathi, B. P.
Baskaran, V.
 
Subject 14 Carotenoid Chemistry
 
Description The combination of carotenoids and doxorubicin (DOX) selectively alters oxidative stress-mediated apoptosis in
breast cancer cells. Primarily, cytotoxic efficiency of carotenoids (β-carotene, BC; lutein, LUT; astaxanthin, AST;
or fucoxanthin, FUCO) either with or without a minimal cytotoxic dose of DOX was evaluated in MCF-7
(0.12 μM) and MDA-MB-231 cells (0.28 μM). The higher cell growth inhibition of BC and/or LUT with DOX was
selected for testing in further cell-based assays. Low-dose DOX significantly enhanced cytotoxicity in carotenoid
(< 5 μM)-treated cells compared to high-dose DOX (> 1 μM) or carotenoid (20 μM) treatment alone. Depleted
glutathione, increased lipid peroxides and increased ROS levels in cells confirmed the cytotoxic effect.
Furthermore, mitochondrial dysfunction, cell growth arrest at G0/G1 phase and caspase cascades as well as upand
down-regulated expression levels of related proteins (p21, p27, Bax, p53, Bcl-2, and cyclin D1) revealed the
synergistic effect of carotenoid and DOX treatment on ROS-mediated apoptosis. These observations demonstrated
increased apoptosis in BC + DOX/LUT + DOX-treated cells due to the pronounced pro-oxidant action.
Interestingly, normal breast epithelial cells (MCF 10A) exposed to similar treatments resulted in non-significant
cytotoxicity. These newly observed mechanistic differences of anticancer drugs on the mitigation of toxicity with
carotenoids may provide insight into the targeting of cancer therapy.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13838/1/Food%20and%20Chemical%20Toxicology%20118%20%282018%29%20675%E2%80%93690.pdf
Vijay, K. and Sowmya, P. R. Rao and Arathi, B. P. and Baskaran, V. (2018) Low-dose doxorubicin with carotenoids selectively alters redox status and upregulates oxidative stress-mediated apoptosis in breast cancer cells. Food and Chemical Toxicology, 118. pp. 675-690. ISSN 0278-6915