Record Details

Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/13873/
https://dx.doi.org/10.1002/lipd.12074
 
Title Dietary Unsaturated Fatty Acids Modulate Maternal
Dyslipidemia-Induced DNA Methylation and Histone
Acetylation in Placenta and Fetal Liver in Rats.
 
Creator Breetha, R.
Ramaprasad, T. R.
 
Subject 17 Fatty Acid Chemistry
 
Description The present study assessed the role of dietary
unsaturated fatty acids in maternal dyslipidemia-induced
DNA methylation and histone acetylation in placenta and
fetal liver and accumulation of lipids in the fetal liver.
Weanling female Wistar rats were fed control and experimental
diets for 2 months, mated, and continued on their
diets during pregnancy. At gestation days of 18–20, rats
were euthanized to isolate placenta and fetal liver. DNA
methylation, DNA methyl transferase-1 (DNMT1) activity,
acetylation of histones (H2A and H2B), and histone acyl
transferase (HAT) activity were evaluated in placenta and
fetal liver. Fetal liver lipid accumulation and activation of
peroxisome proliferator-activated receptor-α (PPAR-α) were
assessed. Maternal dyslipidemia caused significant epigenetic
changes in placenta and fetal liver. In the placenta,
(1) global DNA methylation increased by 37% and DNMT1
activity by 86%, (2) acetylated H2A and H2B levels
decreased by 46% and 24% respectively, and (3) HAT activity
decreased by 39%. In fetal liver, (1) global DNA methylation
increased by 52% and DNMT1 activity by 78%,
(2) acetylated H2A and H2B levels decreased by 28% and
26% respectively, and (3) HAT activity decreased by 37%.
Maternal dyslipidemia caused a 4.75-fold increase in fetal
liver triacylglycerol accumulation with a 78% decrease in
DNA-binding ability of PPAR-α. Incorporation of dietary
unsaturated fatty acids in the maternal high-fat diet significantly
(p < 0.05) modulated dyslipidemia-induced effects in
placenta and fetal liver. Eicosapentaenoic acid (EPA, 20:5n-
3) + docosahexaenoic acid (DHA, 22:6n-3) exhibited a
profound effect followed by alpha-linolenic acid (ALA,
18:3n-3) than linoleic acid (LNA, 18:2n-6) in modulating
the epigenetic parameters in placenta and fetal liver.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13873/1/Lipids%20%282019%29%2053%20581%E2%80%93588.pdf
Breetha, R. and Ramaprasad, T. R. (2018) Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats. Lipids, 53. pp. 581-588. ISSN 0024-4201