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Cell wall remodelling involving galactomannan de-branching influence Agrobacterium tumefaciens-mediated transformation of Coffea canephora somatic embryos.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/13877/
https://doi.org/10.1007/s11240-018-1428-3
 
Title Cell wall remodelling involving galactomannan de-branching
influence Agrobacterium tumefaciens-mediated transformation
of Coffea canephora somatic embryos.
 
Creator Avinash, Kumar
Simmi, P. S.
Giridhar, P.
 
Subject 05 Tissue Culture
04 Coffee
 
Description Direct somatic embryogenesis is favoured over indirect methods for the in vitro propagation of Coffea canephora, as the
frequency of somaclonal variation is usually reduced. Ethylene action inhibitors improve the tissue culture response and thus
silver nitrate (
AgNO3) is used for direct somatic embryogenesis in coffee. It was observed that silver thiosulphate (STS) that
is a more potent ethylene action inhibitor, induced a much robust response in C. canephora cotyledonary leaf explants with
7.49 ± 0.57 and 7.08 ± 0.12 embryos/explant at 60 and 80 μM AgNO3,
respectively compared to 3.3 ± 0.18 embryos/explant at
40 μM AgNO3.
Transient transformation indicated that STS improved the transformation potential of embryos by enhancing
Agrobacterium tumefaciens adherence to surfaces. In vitro adherence assays demonstrated that the cell wall material from
STS-derived embryos provide a better substratum for adherence of Agrobacterium. Furthermore, blocking this substratum
with anti-mannan hybridoma supernatant negatively effects the adherence. The presence of galactose and mannose residues
in the decomposed cellulose fraction of STS treated somatic embryos are indicative of de-branching and re-modelling of
galactomannan in response to ethylene inhibition. Genes of mannan biosynthesis, degradation and de-branching enzyme were
affected to different extents in embryos derived in AgNO3
and STS containing somatic embryogenesis medium. The results
indicate that ethylene-mediated cell wall galactomannan remodelling is vital for improving the transgenic potential in coffee.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13877/1/Plant%20Cell%2C%20Tissue%20and%20Organ%20Culture%20%28PCTOC%29%20%282019%29%20134369%E2%80%93382.pdf
Avinash, Kumar and Simmi, P. S. and Giridhar, P. (2018) Cell wall remodelling involving galactomannan de-branching influence Agrobacterium tumefaciens-mediated transformation of Coffea canephora somatic embryos. Plant Cell, Tissue and Organ Culture, 134. pp. 369-382.