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Differential expression of anthocyanin biosynthesis genes in Daucus carota callus culture in response to ammonium and potassium nitrate ratio in the culture medium.

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Relation http://ir.cftri.com/13882/
https://doi.org/10.1007/s13205-018-1447-0
 
Title Differential expression of anthocyanin biosynthesis genes in Daucus
carota callus culture in response to ammonium and potassium nitrate
ratio in the culture medium.
 
Creator Kirti, R. Saad
Gyanendra, Kumar
Giridhar, P.
Nandini, P. Shetty
 
Subject 04 Biosynthesis
04 Carrot
 
Description Anthocyanins are major water-soluble and dynamic colouring plant pigment present in plant tissues with the high antioxidant
properties. The role of ammonium and potassium nitrate in the culture medium on anthocyanin augmentation is
probed thoroughly, but the mechanism of its biosynthesis continues to be unclear. Hence, the present study was undertaken
to optimise nitrate ratio in the culture medium for anthocyanin augmentation and examination of its biosynthesis pathway
in callus culture of Daucus carota. MS basal medium fortified with various ratio of NH4NO3:
KNO3 was employed to find
their impact on biomass, anthocyanin augmentation and the expression profile of anthocyanin biosynthesis genes in the callus
culture. The data indicated that the highest anthocyanin content (9.30 ± 0.25 mg/100 g FW) was seen in callus grown on
the medium supplemented with 20.0 mM NH4NO3:
37.6 mM KNO3
and the least was seen in the medium which contained
40.0 mM NH4NO3:
18.8 mM KNO3
(2.74 ± 0.27 mg/100 g FW). This indicates an optimal concentration of NH4NO3:
KNO3
ratio is essential to produce a higher amount of anthocyanin in in vitro culture. Meanwhile, anthocyanin biosynthesis genes
were differentially expressed as confirmed by qRT-PCR in the time interval of 5, 10, 15, 20 and 25 days. The transcript levels
of nine anthocyanin biosynthesis genes were increased in the response of varying NH4NO3:
KNO3 ratio in the medium. The
transcript level of early genes PAL, 4CL, CHS and CHI increased by 19.5, 21.0, 16.2 and 9.98-fold, respectively, compared
with control. In addition, late biosynthesis genes LDOX and UFGT resulted in the transcript level of 11.3 and 13.6-fold,
respectively.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13882/1/3%20Biotech%20%282019%29%208431.pdf
Kirti, R. Saad and Gyanendra, Kumar and Giridhar, P. and Nandini, P. Shetty (2018) Differential expression of anthocyanin biosynthesis genes in Daucus carota callus culture in response to ammonium and potassium nitrate ratio in the culture medium. 3 Biotech, 8. pp. 1-11.