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Poly (D, L-lactide-co-glycolide)-phospholipid nanocarrier for efficient delivery of macular pigment lutein: absorption pharmacokinetics in mice and antiproliferative effect in Hep G2 cells.

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Relation http://ir.cftri.com/13886/
https://doi.org/10.1007/s13346-018-0590-9
 
Title Poly (D, L-lactide-co-glycolide)-phospholipid nanocarrier for efficient
delivery of macular pigment lutein: absorption pharmacokinetics
in mice and antiproliferative effect in Hep G2 cells.
 
Creator Arunkumar, R.
Yuki, Manabe
Tatsuya, Sugawara
Takashi, Hirata
Naveen, S.
Baskaran, V.
 
Subject 14 Carotenoid Chemistry
 
Description Lutein has various biological activities, its application in food and pharma industries are limited due to poor aqueous solubility,
stability, and bioavailability. To achieve various benefits, lutein-poly (lactic-co-glycolic acid) (PLGA)-phospholipid (PL)
nanocapsules were prepared. Lutein-PLGA NCs (+PL) were synthesized, characterized and its bioavailability was studied
in vitro and in vivo. The cellular uptake and anti-proliferative activity were analyzed in Hep G2 cells. The mean size and zeta
value of lutein-PLGA NCs (+PL) were 140 ± 6 nm and − 44 mV. The amorphous nature of lutein in PLGA NCs (+PL) was
confirmed by XRD and DSC. In vitro lutein release kinetics showed an initial burst followed by sustainable release up to 86%.
In vitro bioavailability showed 62.7% higher lutein bioaccessibility than lutein in free form. The AUC of lutein after single oral
dose of lutein-PLGA NCs (+PL) revealed 3.91-fold (plasma), 2.89-fold (liver), and 3.12-fold (eyes) higher absorption than the
control (mixed micelles). The IC50 of lutein-PLGA NCs (+PL) in Hep G2 cells at 72 h was 4.5 μM as opposed to 23.4 μM for
lutein in free form. Thus, results reveal that PL added to PLGA NCs helps in enhancing the solubility which in turn resulted in its
better bioavailability and bioefficacy.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13886/1/Drug%20Delivery%20and%20Translational%20Research%202018.pdf
Arunkumar, R. and Yuki, Manabe and Tatsuya, Sugawara and Takashi, Hirata and Naveen, S. and Baskaran, V. (2019) Poly (D, L-lactide-co-glycolide)-phospholipid nanocarrier for efficient delivery of macular pigment lutein: absorption pharmacokinetics in mice and antiproliferative effect in Hep G2 cells. Drug Delivery and Translational Research, 9 (1). pp. 178-191.