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Characterization of Novel Lactobacillus fermentum from Curd Samples of Indigenous Cows from Malnad Region, Karnataka, for their Aflatoxin B1 Binding and Probiotic Properties.

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Relation http://ir.cftri.com/13887/
https://doi.org/10.1007/s12602-018-9479-7
 
Title Characterization of Novel Lactobacillus fermentum from Curd Samples
of Indigenous Cows from Malnad Region, Karnataka, for their Aflatoxin
B1 Binding and Probiotic Properties.
 
Creator Sunil Kumara, S.
Ambika, B.
Venkateswaran, G.
Hariprasad, P.
Gayathri, D.
 
Subject 27 Dairy products
09 Food Microbiology
 
Description Thirty-four isolates of Lactobacillus spp. (LAB) from 34 curd samples were evaluated for their aflatoxin B1 (AFB1) binding and
probiotic properties. Upon characterization, four LAB isolates (LC3/a, LC4/c, LC/5a, and LM13/b) were found to be effective in
removing AFB1 from culture media with a capacity of above 75%. Staining reaction, biochemical tests, pattern of sugar
utilization, and 16s rRNA gene sequence analysis revealed the identity of all the four isolates as L. fermentum. All of them could
tolerate acidic pH, salt, and bile, which promise the use of these probiotic bacterial isolates for human applications. These isolates
showed poor hydrophobicity and higher auto-aggregation properties. All L. fermentum isolates were found susceptible to
gentamycin, chloramphenicol, cefoperazone, ampicillin, and resistant to ciprofloxacin and vancomycin. Results of hemolytic
and DNase activity indicated their nonpathogenic nature. Though all L. fermentum isolates found inhibiting the growth of
Salmonella ebony, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, maximum inhibition was obtained
with isolate LC5/a. Kinetic studies revealed that all four bacteria required a minimum of 2 h to reach stationary phase of AFB1
binding. AFB1 binding ability varied from 66 to 85.2% among these four isolates. Bile (0.4%) was significant (P ≤ 0.05) in
reducing the AFB1 binding property of isolates LC3/a, LC4/c, and LM13/b, while increased AFB1 binding ability was recorded
at acidic pH (2.0). AFB1 binding properties of isolate LC5/a were found least affected by acidic pH and bile. The findings of our
study revealed the higher efficiency of L. fermentum isolate LC5/a in reducing the bioavailability of AFB1 in gut, and additionally,
it improves the consumers’ health by its various probiotic characters. These beneficial characters, L. fermentum isolates,
promise them to use as probiotic formulations alone or in combinations with other beneficial probiotic-bacterial isolates.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13887/1/Probiotics%20and%20Antimicrobial%20Proteins%202018.pdf
Sunil Kumara, S. and Ambika, B. and Venkateswaran, G. and Hariprasad, P. and Gayathri, D. (2018) Characterization of Novel Lactobacillus fermentum from Curd Samples of Indigenous Cows from Malnad Region, Karnataka, for their Aflatoxin B1 Binding and Probiotic Properties. Probiotics and Antimicrobial Proteins. pp. 1-10.