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Comparative Site-Specific N‑Glycosylation Analysis of Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC−MS/ MS Reveals a Distinct Glycan Pattern

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Relation http://ir.cftri.com/13899/
https://dx.doi.org/10.1021/acs.jafc.8b03243
 
Title Comparative Site-Specific N‑Glycosylation Analysis of
Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC−MS/
MS Reveals a Distinct Glycan Pattern
 
Creator Gnanesh Kumar, B. S.
Prasad Mohan, Reddy
Sanjay, K.
 
Subject 07 Enzyme Biochemistry
27 Dairy products
 
Description The N-glycan pattern of lactoperoxidase (LPO) from buffalo and goat milk was analyzed with the corresponding
site of attachment. The enzyme was purified from whey on cation exchange chromatography, proteolyzed using chymotrypsin,
and the resulting (glyco)peptides were directly analyzed on reverse phase ultrahigh performance liquid chromatography coupled
to ESI-Q-TOF MS in tandem mode. N-Glycans such as high mannose, complex, and hybrid types were identified in buffalo and
goat LPO. Among sialylated complex and hybrid types, the terminal Neu5Ac linked to either LacNAc/LacdiNAc found
exclusively in buffalo, whereas Neu5Gc linked to LacdiNAc was predominant in goat LPO. N-Glycans at Asn6 and Asn349 in
buffalo LPO were completely core fucosylated, while these sites in goat LPO showed differential fucosylation. Differential
occupancy was observed at Asn112 with or without nonfucosylated complex and hybrid types, whereas mainly high mannose
glycans were found in Asn222 in both of the LPOs. The presence of glycan isomers in buffalo and goat LPO was also observed.
Despite the presence of distinct complex and hybrid glycans, the common glycosylation features in buffalo and goat LPO were
identified and are comparable with those of bovine LPO. This finding could be useful in exploring the beneficial role of these
glycans as functional ingredients for food products.vvvvvvvvvvvvv
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13899/1/J.%20Agric.%20Food%20Chem.%202018%2C%2066%2C%2011492%E2%88%9211499.pdf
Gnanesh Kumar, B. S. and Prasad Mohan, Reddy and Sanjay, K. (2018) Comparative Site-Specific N‑Glycosylation Analysis of Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC−MS/ MS Reveals a Distinct Glycan Pattern. Journal of Agricultural and Food Chemistry, 66. pp. 11492-11499. ISSN 0021-8561