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β-carotene isolated from the marine red alga, Gracillaria sp. potently attenuates the growth of human hepatocellular carcinoma (HepG2) cells by modulating multiple molecular pathways

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Relation http://ir.cftri.com/13908/
https://doi.org/10.1016/j.jff.2018.11.015
 
Title β-carotene isolated from the marine red alga, Gracillaria sp. potently
attenuates the growth of human hepatocellular carcinoma (HepG2) cells by
modulating multiple molecular pathways
 
Creator Yogendra Prasad, K.
Deepika, U. R.
Sowya Shree, G.
Arpitha, H. S.
Nimish Mol, Stephen
Rangaiah, K.
Ganesan, P.
 
Subject 01 Algae
04 Vitamins
 
Description There remains a remarkable controversy on the anti-cancer stand of β-carotene specifically when high concentrations
are used. This study aimed to examine the growth inhibitory effect of the lower concentrations of β-
carotene in HepG2 cells. Beta-carotene purified from Gracillaria sp. inhibited viability of HepG2 cells in a
concentration-dependent manner. This growth inhibitory effect was well correlated with increased apoptotic cell
death. Apoptosis induction by β-carotene was associated with increased caspase-3 activity and reduced mitochondrial
membrane potential. Western blot analysis showed a significant decrease in the expression of Bcl-2,
PARP, and NF-kB protein, and an increase in the protein expression of Bax. Beta-carotene also blocked the
activation of intracellular growth signaling proteins, Akt and ERK1/2. Further, it down-regulated the expression
of endogenous antioxidant enzymes, SOD-2 and HO-1, and its transactivation factor, Nrf-2. The current study
emphasizes the role of β-carotene as an effective inhibitor of the growth of hepatocarcinoma cell.
 
Publisher Elsevier
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13908/1/Journal%20of%20Functional%20Foods%2052%20%282019%29%20165%E2%80%93176.pdf
Yogendra Prasad, K. and Deepika, U. R. and Sowya Shree, G. and Arpitha, H. S. and Nimish Mol, Stephen and Rangaiah, K. and Ganesan, P. (2019) β-carotene isolated from the marine red alga, Gracillaria sp. potently attenuates the growth of human hepatocellular carcinoma (HepG2) cells by modulating multiple molecular pathways. Journal of Functional Foods, 52. pp. 165-176. ISSN 1756-4646